نتایج جستجو برای: polymerase chain reaction (pcr)

تعداد نتایج: 810995  

2009
Paul C Winter

The polymerase chain reaction (PCR) is a powerful and widely used technique that has greatly advanced our ability to analyze genes. Genomic deoxyribonucleic acid (DNA) present in cells contains many thousands of genes. This makes it difficult to isolate and analyze any individual gene. PCR allows specific DNA sequences, usually corresponding to genes or parts of genes, to be copied from genomic...

پایان نامه :وزارت علوم، تحقیقات و فناوری - دانشگاه کردستان - دانشکده کشاورزی 1392

بیماری¬های ویروسی ناشی از نپوویروس¬ها از مخرب¬ترین بیماری¬های ویروسی مو در سراسر جهان به حساب می¬آیند که با نماتدهای خانواده longidoridae منتقل می¬شوند. اغلب این ویروس¬ها دارای میزبان¬های طبیعی متعددی در بین گیاهان زینتی و غیر زینتی، درختان میوه و علف¬های هرز می¬باشند. پیکره اعضای این جنس دو بخشی، جورقطر و به قطر حدود 30 نانومتر می¬باشد. غلظت این ویروس¬ها در گیاه و به ویژه تاک پایین است و در نت...

Journal: :jundishapur journal of health sciences 0
siavash maktabi department of food hygiene, faculty of veterinary medicine, shahid chamran university, ahvaz, ir iran; department of food hygiene, faculty of veterinary medicine, shahid chamran university of ahvaz, p. o. box: 61355145, ahvaz, ir iran mehdi pourmehdi department of food hygiene, faculty of veterinary medicine, shahid chamran university, ahvaz, ir iran mehdi zarei department of food hygiene, faculty of veterinary medicine, shahid chamran university, ahvaz, ir iran amir ali fooladgar faculty of veterinary medicine, shahid chamran university, ahvaz, ir iran

conclusions l. innocua is not a pathogen, but the presence of the bacterium could be an indicator of probable contamination with l. monocytogenes. moreover, there is a potential risk to public health from the consumption of raw or undercooked burgers, which may increase the possibility of the acquisition of resistance to antibiotics. objectives the aim of this study was to evaluate the occurren...

Journal: :بینا 0
علیرضا مالکی ar maleki زاهدان- بیمارستان چشم پزشکی الزهرا (س) تقی ناصرپور فریور t naserpoor farivar زاهدان- بیمارستان چشم پزشکی الزهرا (س) علی توللی a tavallali زاهدان- بیمارستان چشم پزشکی الزهرا (س)

purpose: to ascertain whether the use of nested-pcr (nested polymerase chain reaction) technique leads to more rapid and accurate diagnosis of bacterial endophthalmitis. methods: the aqueous humor and vitreous samples of 24 eyes of 24 patients with bacterial endophthalmitis were evaluated by microscopy, diagnostic aerobic culture, and nested- pcr to detect the infectious agent. results: aerobic...

Journal: :international journal of molecular and clinical microbiology 0
mina eghbali young researchers and elite club, tonekabon branch, islamic azad university, tonekabon, iranسازمان اصلی تایید شده: دانشگاه آزاد اسلامی تنکابن (islamic azad university of tonekabon) fariba sadeghi department of medicine, tonekabon branch, islamic azad university, tonekabon, mazandaran, iranسازمان اصلی تایید شده: دانشگاه آزاد اسلامی تنکابن (islamic azad university of tonekabon) reza golijani moghadam department of genetics, tonekabon branch, islamic azad university, tonekabon, mazandaran, iranسازمان اصلی تایید شده: دانشگاه آزاد اسلامی تنکابن (islamic azad university of tonekabon)

today, it has been confirmed that the viral infections play an important role in premature delivery and abortion. this study was carried out to determine the frequency rate of papilloma virus types 16 and 18 in pregnant women and their relationship with premature delivery and abortion. in this study, vaginal secretions were collected from pregnant women who had been referred to the women clinic...

Journal: :molecular and biochemical diagnosis (journal) 2014
masood ghane negin naghdi

background: acne vulgaris is an inflammatory chronic disease of pilosebaceous unit. one of the most important factors playing a role in occurrence of acne is presence of propionibacterium acnes. with the aim of molecular identification of the p. acnes from the acne vulgaris lesions, current research was carried out. methods: in this study, contents within the lesions was collected from 70 patie...

Journal: :iranian journal of pathology 2015
masoumeh salehpour naser tayyebi meibodi roghayeh teimourpour adel ghorani-azam samaneh sepahi

background &objective: breast cancer is the most common female malignancy. detection of dna of human papillomaviruses (hpvs) in breast carcinomas suggests that the virus may play a role in the pathogenesis of this disease. the aim of this study was to evaluate the frequency of hpvs genotypes 6, 11, 16, 18 and 31 in paraffin-embedded tissue samples of invasive breast carcinomas. methods: thr...

Journal: :jundishapur journal of microbiology 0
aida gholoobi department of modern sciences and technologies, school of medicine, mashhad university of medical sciences, mashhad, ir iran ali masoudi-kazemabad department of biology, science and research branch, islamic azad university, tehran, ir iran mojtaba meshkat department of biostatistics, mashhad branch, islamic azad university, mashhad, ir iran zahra meshkat antimicrobial resistance research center, faculty of medicine, mashhad university of medical sciences, mashhad, ir iran; antimicrobial resistance research center, mashhad university of medical sciences, p.o box: 9196773117, mashhad, ir iran. tel: +98-5118012453, fax: +98-5118002287

conclusions: pcr method is a valuable, cost-effective and alternative tool for quick diagnosis of active tuberculosis in different clinical specimens. results: the concordance rate between the three sets of primers was calculated and is6110/buffer pcr method showed good agreement with the lj culture method (κ = 0.627, p < 0.0001). the sensitivity of is6110/buffer pcr was 58.33%, with specificit...

Journal: :archives of razi institute 2016
b. mosallanejad n. vakili r. avizeh m.r. seyfiabad shapouri m. pourmahdi

canine parvovirus type 2 (cpv-2) is one of the most common viruses responsible for acute hemorrhagic enteritis in dogs. a rapid and accurate diagnosis of cpv-2 infection is especially important in kennels in order to isolate infected dogs. the aim of the present study was to compare two laboratory tests i.e., polymerase change reaction (pcr) and immunochromatography assay (ica) most commonly us...

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